Label the parts of the compound microscope below. M:\My Documents\work\Bio 114\Bio 114 lab\Lab 7 whole lab.doc - 3 - Study the cells in this area and compare them to Fig. NAME ID INTRODUCTION. The electron beam from an electron gun is directed towards the specimen and steered using an arrangement of apertures and magnetic lenses. By observing, drawing, and classifying protista, we learned about the cell structure and movement patterns of these one-celled organisms. 2. 3. V. Remove the distilled water using filter paper. The light microscope is a very powerful tool for understanding the structure and function of tissues, and it is widely used in biomedical science courses, as well as in research and diagnostic laboratories. Turn it on and make sure that the light comes on (it may take a second or two to warm up). Explain why the light microscope is also called the compound microscope. Pre-lab: 1. The main aim of this section is to look at cells. State TWO procedures that should be used to properly handle a light microscope. 2. b) To understand what Microscopy is, and how it can be used to observe . 1. View lab report paper.pdf from AA 1Experiment 1: Basic Techniques in Microscopy_DB014 Marks Content Title: Experiment 1: Basic Techniques in Microscopy Objectives: 1. Many kind of instruments, ranging from the hand lens to the electron microscope, are effective magnifying devices. Answer lab questions 1-5 before beginning experiment 3. The lower the power, the easier to focus. Procedure: Get a glass slide and cover slip for yourself and make sure they are both thoroughly washed and dried. The main objectives in this lab were the following: the . Wearing goggles or a lab coat will help towards preventing any accidents. This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The lab report should be divided into sections (see below) and written as clearly and concisely as possible. Record the time, in seconds, how long the pill bug remains curled up. Note the type of asexual spores produced and on what they are borne. 1 - 6) and the procedures (pp. Ensure that the details you fill in Cheek And Onion Cell Lab Report Introduction is updated and correct. Area Z was then located . Embedded quizzes to help students master science content. A response is required for each item marked: (#__). This experiment is an incredible biology lab for students in Upper Elementary through High School. In addition, we needed to look at the contrasts of some specimens in this lab. To make a slide, tear a 2 -3" long piece of Scotch tape and set it sticky side up on the kitchen table or other work area. Laboratory Experiments Microbiology Labs Microbiology Labs II Lab 1: Introduction to the Microscope and Comparison of Sizes and Shapes of Microorganisms . Add a drop of purple stain (specific for animals) and cover with a cover slip. 3. VI. The outermost and the thinnest layer of the onion was peeled. Purpose: To familiarize the student with the care and use of the light microscope. A14-A15). If the microscope light does not turn on, check with your teacher. After making sure that the water drop is larger than the slice of cork, use the tweezers to place the slice of cork on the water. Remember microscope is a costly, precision instrument and must be handled with caution. Lab 1: Introduction to the Microscope and Comparison of Sizes and Shapes of Microorganisms is shared under a not declared license and was authored, remixed, . This report presents an introduction to the compound microscope followed by a number of procedures. This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. microscopic methods involve examination of soil samples under a light microscope by using simple stains such as phenol aniline blue and fluorescent stains such as fluorescein isothiocyanate (fitc), acridine orange, rhodamine (fluoresces red), europium chelate (europium (iii) thenoyltrifluoroacetonate), dapi (4'-6'-diamidino-2-phenyl-indole), Use material from Section 18.1 of your text to label the condenser, objective, and ocular lenses in the diagram of the compound light microscope in Figure Make sure that the microscope is working properly before starting the experiment. We want to examine, to know the characteristic and to differentiate human cheek cells and onion skin cells. Because biological objects can be very small, a microscope is often used to view them. Experiment 3 - Metallographic Observations Background Materials engineers can predict the general behavior of materials by observing their microstructure. In each protocol you will find a list of materials necessary, and step-by-step instructions on how to prepare your sample. Repeat this four more times. 4- To correctly use the microscope to see perfectly clear images of different specimen. Switch off the light source after finishing the experiment. tensile, fatigue, 1 Microscope (Visual Observation) Experiments Prepared by Dr. C. S. Tritt September 25, 2007 2 Purpose Provide students with the opportunity to practice: the scientific method, use of literature resources record keeping (logbooks), report writing, visual observation and microscope use. Compare your microscope with Figure 2 on the next page. LAB 3 - Use of the Microscope Introduction In this laboratory you will be learning how to use one of the most important tools in biology - the compound light microscope - to view a variety of specimens. Explain what this means. 2. compound microscope. 3.5 Metallurgical Microscope. Add a drop of iodine solution on the onion membrane (or methylene blue) Gently lay a microscopic cover slip on the membrane and press it down gently using a needle to remove air bubbles. Lay it out flat on your working surface and slice about a 1" section crosswise out of the center using a sharp knife. The microscope is marked by its accuracy and exactness. Using high power, count the number of nuclei visible in a high power field and record your answer in assignment question . In this experiment 1, we are using optical microscope to observe onion skin cells and human cheek cells. Thoroughly scrub the skin of one fingertip with a cotton swab and rubbing alcohol. You can also hold a tissue at the edge of the slide to absorb excess stain and close any bubbles. a. Familiarize yourself with the microscope, run the tutorial and examine the parts you will be working with. Place the slide on the microscope, with 4 x or 10 x objective in position and find a cell. Using the light microscope the objects can be enlarged or magnified with a convex lens that bends light rays by refraction. Contact us now. 2. First, check it for bubbles- if you notice any, grab your pipette, and using the bulb end, lightly tap the slide until the bubble disperses. Using your microscope, observe a prepared slides of Penicillium. Remove the single layer of epidermal cells from the inner (concave) side of the scale leaf (The thinner the better). Source of error . worksheet. The structure of a typical lab report includes the following sections: Aim and Hypothesis - Why you conducted the practical work. Using the course focus and low power, move the body tube down until the "e" can be seen clearly. Conclusion: Follow the directions on how to write a laboratory report. Understanding the capabilities and limitations of the light microscope is important if one is to get the best results from microscopy. To identify the parts of a compound light microscope. This lab showed me what materials and parts of the microscope I should use when examining an object. Objectives: a) To Learn the preparation of specimen for microscopic observation. Carefully lower the cover slip and ensure no air bubbles are . Using a Compound Microscope Lab Report E Period Abstract The purpose of this lab is to learn about the parts and uses of a compound microscope. Identify the parts on your microscope and determine the function of each part. bottom with the root cap, then the zone of cell division next is the zone of cell elongation, and at the top of the root is the zone of differentiation In order to examine cells in the tip of an onion root, a thin slice of the root is placed onto a microscope slide and stained so the chromosomes will be visible Then we observed under different . 6 - 17) for each part of the experiment, and then answer the prelab questions at . I. Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds. Switch on the Wizard mode on the top toolbar to get extra recommendations. Fill each fillable field. Methylene blue stains negatively charged molecules in the cell, including DNA and RNA. Draw the structure of the onion cells as observed under microscope. 8-1 until you can easily recognize cells in interphase, prophase, metaphase, anaphase and telophase. 4. Besides the crystallographic nature of a material, imperfections inside a material have an even greater influence on the mechanical properties, i.e. 190-191 and appendix pp. 250+ Web-based simulations that can be played on laptops and tablets without installing any software. The microbes under the microscope were drawn out and labelled. The SlideShare family just got bigger. The experiment setting was kept as sterile as possible when conducting the experiment. Lab Report about Simple Staining of Microbes. (We will observe blood cells in a future lab!!) Next, sprinkle a few grains of salt or sugar in the middle of the sticky part of the slide. The slide was then placed on the microscope. Read Dragonfly (pp. Determine what happens to the image of a specimen in the microscope. Ruler Procedures . Methylene blue, a simple staining component, was used to stain the slide in order to see the different microbes and determine their cellular shape. In this experiment, a small bit of an onion was cut off. In an actual laboratory setting, there are certain important steps that are not necessarily applicable in a virtual lab. Record a description of the pill bugs response. Add a drop of methylene blue solution and place a coverslip on top. Concentrated methylene blue is toxic if ingested. Microscopic Observation of Unicellular and Multicellular Organisms Prelab Assignment Before coming to lab, read carefully the introduction (pp. Name the parts of the microscope describe the function of each. The directions in the lab handout said to place the letter "e" on the slide so that the "e" was right side up and to ake sure the letter was positioned directly over the hole where the light was coming in. 3. by Unknown Author is licensed under. To handle microscope Afterwards, carefully mount the prepared and stained onion cell slide onto the microscope stage. 3. Draw what you see in the space below. The directions said to use the scanning objective, which is the shortest one and use the coarse knob to bring the "e" into focus. 2. Study of Compound Microscope. The purpose for completing this lab was to observe how microscopes function. If you can't see the video, please turn off your adblockers, as they also block our video feed. Teacher dashboard to automate grading and track student progress. Fold over about " of the tape on each end to form finger holds on the sides of the slide. Images observed under the light microscope are reversed and inverted. The purpose of a microscope is to see inside of organisms and cells- to see what is invisible to the naked eye [lab manual, 2014]. 3. Place a drop of 5% (w/v) sucrose solution at a side of the cover slip and draw the solution across the epidermal layer by placing filter paper on the other side of cover slip. 1. 2. To help search for potential alterations, the MICROSCOPE experiment tracked the motion of nested metal cylinders a 300-gram titanium outer cylinder and a 402-gram platinum inner one as . Title Page The title page of the lab report should be blank except for the Title of the Lab Report, Date Due, Your Name each centered on a separate line. Microscope is a device which is used to magnify the smallest entities which is not visible from our naked eye. A science lab report is a structured way of communicating the outcomes of your practical work. Open a lancet to expose the sharp point (about 3 mm long). EXPERIMENT -1 1. Aim of the experiment: Objective of Study: 1- To identify the different parts of the microscope. * Unused roots can be stored in 70 percent alcohol. Last modified by: Tami Created Date: 9/28/2012 7:10:00 PM Company 3. Search: Onion Cell Lab Report Results. 8 pages, 3512 words. Objective: To examine the nature of inhomogenities, flow lines and welded sections of a metal by unaided eye (or with a low-power microscope). Then, starting at one of the short ends (the edges that you did not cut), tightly roll the leaf section. LAB CREDITS EARNED 0 1 Then view at higher magnification. Conclusion Questions: 1. Filed Under: Reviews. Materials: Microscope. 4. We list here a few live samples with interesting cell types that we have tested and recommend. however, for the sake of this lab a compound/binocular microscope was useda microscope that has two lenses between the eye and the slide. Three reasons for beginning with the scanning objective are: 1. 1 Introduction Scanning Electrons Microscope (SEM) is a powerful characterization tool for materials of various sizes and shapes, and is extremely important in the study of nano-scale structures. Version 42-0027-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report.The Lab Report Assistant is simply a summary of the experiment's questions diagrams if needed and data tables that should be addressed in a formal lab report. The average microscope has a resolving power of up to 0.2 micrometers. Plain glass microscope slide Slide cover slip Methylene blue stain Q tip or flat toothpick Instructions Scrape the inside of your cheek with the Q-tip and wipe it on to the center of the slide. Handling any glassware for example beakers or petri dishes with care. There are also some simple experiments that you can do . Format of a Scientific Lab Report 1. Place the single layer of onion cell epithelium on a glass slide. Compound Microscopes. It is also to learn how to properly use and take care of the microscope. b. Binocular model x Eye pieces: 10X, 15X & 2 OX Lab 3: Microscope and Cells (revised fall 2009) Lab 3 - Biol 211 - Page 1 of 26 Lab 3. 2. Touch a blotting paper on one side of the slide to drain excess iodine/water solution, Place the slide on the microscope stage under low power to observe. "Light Microscopy Lab Report" Get High-quality Paper helping students since 2016 In the light microscopy, the lenses are arranged so that the images of objects too small to be seen with the naked eye can become highly magnified, so the apparent size can be increased, and their minute detail can be revealed. Your grade for the lab 1 report (1A and 1B combined) will be the fraction of correct responses on a 50 point scale[(# correct/# total ) x 50]. Flow Lines iii. the aims of observations in this experiment were to specify different eukaryotic cell structures of animal and plant cells, to determine their morphological properties which can be observed under a light microscope, to estimate the sizes of animal and plant cells and, to determine and compare the structural and basic differences between gram (+) Light microscope is mainly used in the lab activities. Remove any excess solution by allowing a paper towel to touch one side of the coverslip. 3. Microscopy that was used were magnification, slide preparation, and staining. Microscopy Biology Lab Exercise & Report Subject: How to Use a Compound Light Microscope Author: Tami Port Keywords: microscopy lab exercise, biology lab exercise microscope,instructions on how to use microscope Description: Biology lab exercise on basic use of microscope. Make sure that you do not fold it over or wrinkle it. Results The invention & evolution of the microscope has been an ongoing process since the Middle Ages, when the first convex magnifying lenses were introduced. 3- To learn the different objective powers of the microscope. This dye is toxic when ingested and it . Access the Virtual lab by clicking on the link that says "the virtual scope". Click on the orange Get Form option to start filling out. 2. These failure tests included tensile tests, impact tests, and fatigue tests. Microscopes Heat from the microscope can be harmful to the Daphnia, keeping the brightness low won't kill any Daphnia. Check out the video of this experiment! 4. . 3 Specific Requirements Hold the cover slip at an angle to the slide so that one edge of it touches the water drop. Contrast is defined as being able to see different parts of the specimen at hand. Observe the cheek cells under both low and high power of your microscope. 2. You have two objectives to fulfill by the end of this lab: Properly use and focus a microscope from a low power to a high power objective lens. Experiment: i. Sulphur Printing ii. 1. IV. Include the date to the template with the Date feature. Define resolving power, field of view, depth of field (focus),working distance. Lab Report on Basic Microscope. Gently roll and rub the toothpick onto the top of a glass slide in an area that will be visible through the microscope. In this lab, we adjusted the resolution on the microscope to have a better look at the specimens that were observed. Just for you: FREE 60-day trial to the world's largest digital library. Plug the microscope in at your lab desk. To observe and draw the letter "e" as it appears under a microscope. Slide. Introduction Look through the eye piece and bring. 2. Focus first using the yellow-striped 10X objective (100X magnification) and then rotate to the 40X objective (400X magnification). The visibility of the microscope depends on the resolution. Turn the nose piece to bring the low power objective lens in a line with the body tube. 82510 Microscope Lab 2-5 Name_____ Exercise #2 Finding & Focusing Report Sheet All microscope observations begin with the Scanning (4X) objective. Focusing instructions when using the 10X objective can be found in Lab 1. Lab 4 - Lab Report on Acid-Fast Stain, Structural Stain, and Special Media for Isolating; Exam 1 - Lecture notes 1-7 . 2- To know the functions of the different parts of the microscope. Problem, Hypothesis and Prediction In this lab experiment, Hannah was to analyze an onion root tip under a microscope to observe the mitotic stages that occur in the cells of onion root tips. Objectives: At the end of this exercise the student should be able to: 1. Wear gloves and do NOT allow children to handle methylene blue solution or have access to the bottle of solution. Microscope and supplies to make slides Magnifying Glass will work too if you don't have a microscope . Library of learning resources, lab reports, videos, theory pages, graphics and more. The experiment setting was kept as sterile as possible when conducting the experiment. Method - How you conducted the practical work and how any data processed. Then, answer the following questions and look at the four slides using the virtual microscope (see directions below). II. Welded Sections Kasrga, 6 i. Sulphur Printing: The purpose of this experiment is to examine the impurities that may exist in steel products. Then calculate the average response time. We also made drawings of our observations using light and dissection microscopes to practice proper microscopy skills, including making wet-mount slides and cell sizing. Glass ware breaking. For example, your Introduction could begin, "In this laboratory experiment, the ability of a lab microscope to differentiate between different species of single-cell organisms was tested." Methods and Results should all be written in the past tense, since you'll be summarizing actions that you've already performed as part of the lab. 2. Quickly puncture the cleaned fingertip, put the lancet down, and gently squeeze the finger until a small drop of blood forms on the fingertip. Today, you will use that knowledge to determine how a microscope works. Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Clean the prepared slide and place it on the stage. Lab 2 Microscopy. 3. Scanning Electron Microscope Experiment Results Good Essays 853 Words 4 Pages Open Document The purpose of this lab was to implement the use of a Scanning Electron Microscope (SEM) to examine and analyze metal samples that were subject to different failure tests. LAB REPORT EXPERIMENT 4: MICROSCOPY DATE PERFOMED: 17 OCTOBER 2019 AS1201F NO. How to make the microscope slide: Before you begin, make sure the leaf is clean and dry. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. Hold the coverslip or another slide with one end flush on the slide and gently wipe the edge of the coverslip over the scrapings. Onion Root Tip Lab Report Hannah Scott Biology Ms. Carpenter February 25, 2013 . This allows for magnification to occur and adequate lighting, that work hand-in-hand . Using The Microscope Laszlo Vass Ed.D. Place the scope back in its original space in the cabinet. Wipe the back side of the slide using tissue paper. Spelling and grammar count. AIM -: Preparation and study of the Micro Structure of pure metals like Iron, Copper and Aluminum. Upload this worksheet to the Canvas site . Examine the onion cells through the microscope. 3. 26, 1064-1065) or Parrot (pp. Focus the mirror for maximum illumination of the object. The lower the power, the greater the field (area) of view. You will also use a slightly different type of light microscope called a stereoscopic dissecting microscope. Using the pipette, place one drop of water on the slide. Results - What was the data, process or product . 2. Turn on the microscope and place the slide on the stage; making sure the "e" is facing the normal reading position (see the figure above). Cover the sample (root tip) with a coverslip and gently press the coverslip down, then examine the slide under the microscope starting with low magnification * For this experiment, a properly prepared slide should appear light pink due to the stain to almost colorless.